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Objective: To observe the effect of Shoutaiwan and Shaoyao Gancao Tang on recurrent spontaneous abortion due to spleen and kidney Qi deficiency. Method: Totally 80 eligible patients were randomly divided into the control group and the treatment group, with 90 cases in each group. The control group was treated with lymphocyte immune; the Shoutaiwan and Shaoyao Gancao Tang combined with lymphocyte immune was applied in the treatment group. Coagulation function[the part plasminogen activator inhibitor-1 (PAI-1), fibrinogen (FIB), type plasminogen activator (t-PA), prothrombin time (PT), D-dimer (D-D) and tissue factor (TF), fibrin peptide A (FPA), fibrinogen (Fig), Plasminase-antifibrinolytic complex (PAP), glycoprotein platelet particle-140 (GMP-140), thrombin-antithrombin complex (TAT)], serum closed antibody (CD3+,CD4+,CD8+,CD4+/CD8+), inflammatory factor interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), interleukin-10 (IL-10)]were scored after the treatment. The rates of birth and adverse reactions in each group were compared. The traditional Chinese medicine(TCM) syndrome differentiation of abortion spleen and kidney Qi deficiency was scored. Result: 5 cases were lost during the study period. The term delivery rate was 92.0%in the treatment group, which was higher than 73.6%in control group (PP+, CD4+, CD4+/CD8+), inflammatory factors (IFN-γ,TNF-α,IL-1β) and TCM syndrome scores were significantly lower in the treatment group than that in control group (P+), inflammatory factors IL-10 were significantly increased compared with the control group (PPConclusion: Shoutaiwan and Shaoyao Gancao Tang have a remarkable effect on recurrent spontaneous abortion case by spleen and kidney Yang deficiency.
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Objective: To optimize the preparation technology of geniposide ethosome spray (GES) and to evaluate the regulation of its nasal mucosa permeability in vitro and nasal ciliotoxicity. Methods: Geniposide ethosomes were prepared by ethanol injection method. An central composite design-response surface method was used to optimize the related factors and technical parameters in the preparation of geniposide ethosomes with entrapment efficiency as evaluation index. Their physical properties were evaluated by the transmission electron microscope and photon correlation spectrometer. The isolated pig nasal mucosa was used to investigate the regulation of GES nasal mucosa permeability in vitro. The accumulated permeation amounts of geniposide aqueous solution, geniposide liposomes, and geniposide ethosomes were compared. In situ toad palate model was established to evaluate the effects of geniposide ethosomes on persistent vibration duration of toad palate cilia, so as to evaluate the cililary toxicity. Results: The average encapsulation percentage, particle size, drug loading, and Zeta potential of geniposide ethosomes were (65.80 ± 2.53)%, (173.40 ± 71.02) nm, (5.25 ± 0.15)%, and (-42.50 ± 8.27) mV, respectively. The accumulative permeation amount of geniposide ethosomes in 300 min was 23.39 μg/cm2, which was about 2.17 times of liposomes and 11.03 times of geniposide aqueous solution. Furthermore, the GES showed less mucociliary toxicity. Conclusion: The optimized formulation and preparation technology of geniposide ethosomes are rational. GES could significantly increase the mucosa permeability of geniposide and could be used for nasal administration.
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<p><b>OBJECTIVE</b>To study the antioxidant constituents from the roots of Securidaca inappendiculata.</p><p><b>METHOD</b>The bioassay-guided isolation of antioxidant constituents was carried out by the column chromatographic techniques. The combination of IR, MS, NMR and 2D-NMR spectroscopics methods was used to identify their structures.</p><p><b>RESULT</b>Two new xanthones, 1, 2, 5-trihydroxy-6, 8-dimethoxy-9H-xanthen-9-one(1), 1, 5-dihydroxy-2, 6, 8-trimethoxy-9H-xanthen-9-one (2), along with seven known ones, 3, 8-dihydroxy-1, 4-dimethoxy-9H-xanthen-9-one(3), 4, 6-dihydroxy-1, 5, 7-trimethoxy-9H-xanthen-9-one(4), 7-hydroxy-1, 2, 3, 8-tetramethoxy-9H-xanthen- 9-one(5), 1, 7-dihydroxy-9H-xanthen-9-one(6), 4-hydroxy-3, 7-dimethoxy-9H-xanthen-9-one(7), 1,7-dimethoxy-9H-xanthen-9-one(8) and aucuparin(9), were isolated from the roots of S. inappendiculata.</p><p><b>CONCLUSION</b>Compounds 1 and 2 were new xanthones, and compound 3 was isolated as a natural product for the first time, and compounds 4 and 6 were isolated for the first time from this genus. The antioxidant activities of all compounds were evaluated by ABTS, FRAP and DPPH assays respectively. Compound 9 showed significant activity by the ABTS and FRAP assays. Compound 1 showed significant activity with IC50 value of 0.31 mg x L(-1) in DPPH assay. Scavenging capacity of all compounds determined by all assays were well correlated between ABTS and FRAP assay (r = 0.9555).</p>
Subject(s)
Antioxidants , Chemistry , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Securidaca , Chemistry , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Infrared , Xanthones , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the relation between the 389A/G polymorphism in the human beta 1-adrenergic receptor and acute myocardial infarction (AMI).</p><p><b>METHODS</b>Polymerase chain reaction amplification and restriction fragment length polymorphism analysis were used to detect the genotypes of 150 patients with AMI and 150 age- and sex- matched control subjects, and relative clinical data were obtained. A case-control study and multiple Logistic regression analysis were performed to assess the association between 389A/G polymorphism and AMI.</p><p><b>RESULTS</b>The distributions of the genotypes and allele frequencies were significantly different between two groups (P< 0.01). The prevalence of the A allele was significantly higher in patients with AMI than in control subjects. In the multivariate regression analysis, the 389A/G polymorphism (OR: 2.88, 95%CI: 1.70-4.88, P< 0.01), smoking(OR: 2.72, 95%CI: 1.52-4.88, P< 0.01), hyperlipidemia (OR: 2.85, 95%CI: 1.68-4.86, P< 0.01), diabetes mellitus(OR: 2.38, 95%CI: 1.27-4.47, P< 0.01) and hypertension (OR: 2.00, 95%CI: 1.62-3.45, P< 0.05) were independent risk factors of AMI.</p><p><b>CONCLUSION</b>The 389A/G polymorphism in the human beta 1-adrenergic receptor is associated with AMI and is an independent risk factor of AMI.</p>